Narcolepsy Essay -- Disorder Sleep Sleepy Health Essays

Narcolepsy Lethargy, regardless of whether because of rest apnea, overwhelming wheezing, idiopathic hypersomnolence, narcolepsy or a sleeping disorder from any number of rest related scatters, compromises a huge number of Americans' wellbeing and financial security (1). Maybe to some degree most worried of these scatters are those that permit rest without having any power over when it happens-idiopathic hypersomnolence and narcolepsy. The two are firmly related in that both reason people to nod off without such control, yet narcolepsy happens with no dreaming during rests (2). For a considerable length of time, narcoleptic individuals have been nodding off in corners, worried, as they have given various endeavors to attempt to remain engaged and conscious. Be that as it may, other than the inordinate exhaustion that individuals experience, there most likely should be more that can be related with causing such tiredness among individuals at an uncontrolled level. There may particularly not be an explanation including the I-capacity of the cerebrum, as individuals don't know about when essentially they will fall into their profound rest. Narcolepsy has been clinically characterized as a ceaseless neurological issue that includes the body's focal sensory system (CNS). The CNS is fundamentally similar to a parkway of nerves that conveys messages from the mind to different pieces of the body. Along these lines, for individuals with narcolepsy, the messages about when to rest and when to be conscious some of the time hit barriers or reroutes and show up in an unlucky spot. This is the reason somebody who has narcolepsy, not oversaw by meds, may nod off while having supper or occupied with social exercises or even now and again when they are so centered around being wakeful, yet they can't be because of their narcoleptic nature. Much of the time, be that as it may, conclusion... ...r individual sorrow. Works Cited: 1)Sleep Apnea, Snoring, Narcolepsy, Insomnia and Other Causes of Daytime Fatigue http://members.tripod.com/~sleephealth/sleepdisorderssleepapnea.html 2)Better Sleep Now! http://www.dreamdoctor.com/better/narcolepsy/6.shtml 3)Center for Narcolepsy: Symptoms and Diagnosis http://www.med.stanford.edu/school/Psychiatry/narcolepsy/symptoms.html 4)Living With Narcolepsy http://www.sleepfoundation.org/distributions/livingnarcolepsy.html 5)Sleepnet.com Apnea Forum http://www.sleepnet.com/apnea18/messages/434.html 6)Seratonin: The science of Well-Being http://www.angelfire.com/hello there/TheSeer/seratonin.html 7)Sleep Channel: Narcolepsy http://www.sleepdisorderchannel.net/narcolepsy/treatment.shtml 8)Sleep: Alternative and Integral Therapies http://www.holistic-online.com/Remedies/Sleep/sleep_ins_food-and-diet.htm

Wireless LAN Technology Coursework Example | Topics and Well Written Essays - 2500 words

Remote LAN Technology - Coursework Example WLAN TechnologyThe IEEE 802.11 WLAN innovation is a system get to innovation utilized in arrangement of availability between remote focuses and wired frameworks of a system. It is commonly known as the Wi-Fi which is a total of various advances. Intel has been a productive supporter of the IEEE 802.11 measures whose primary work is fixated on improving QoS, expanding transmission extend just as paces, and including new abilities. The IEEE 802.11 WLAN innovation is a broad family made up of various advances which are attached to an assortment of conventions that have been developing since the presentation of IEEE 802.11 in 1999 [2, 3]. The conventions incorporate IEEE 802.11a, IEEE 802.11b, IEEE 802.11n, IEEE 802.11g, IEEE 802.11e, IEEE 802.11i, IEEE 802.11u, IEEE 802.11w, IEEE 802.11s, IEEE 802.11ac, and IEEE 802.11ad [2]. One principle distinction between these measures is information rate [4]. Some IEEE 802.11 conventions are coordinated into systems administration chipsets to fram e IEEE 802.11 WLAN advances which are accessible for procurement. These conventions incorporate IEEE 802.11a, IEEE 802.11b, IEEE 802.11n, and IEEE 802.11g [2, 4]. Before buying any chipset, the innovation underneath it must be inspected. This includes looking at their exhibition dependent on the IEEE 802.11 conventions incorporated into them. The distinctions in the WLAN innovations can be classified dependent on recurrence, average information rate, most extreme information rate, and range. For example, IEEE 802.11b was presented in September 1999.

Ancient Chinese secret, eh

Ancient Chinese secret, eh On this otherwise lovely three-day weekend, my suitemate Jeremy 09 found himself stricken with a case of influenza, exhibiting such symptoms as nasal congestion, a sore throat, and a mild fever. So, he called up a friend from high school, who happens to be a pre-med (DING!) at Harvard (DING!) who recommended the ancient Chinese secret (double DING!) of dissolving salt in hot water to make a solution strong enough to taste, then snorting said solution up your nose. Fortunately, prior to subjecting himself to this remedy, Jeremy came to me to ask if it had any scientific merit from the perspective of a chemical engineer. Well, if you snort a salt solution up your nose, I guess, by osmosis, the cells of your nose should lose water to the higher-concentration salt solution, which might then dissolve whatevers blocking your nose. Apparently this was a satisfactory explanation for Jeremy: Imagine what happens to the foul cocktail of snot, mucus and salty water goes after it passes through your nose. Better yet, dont. Although Jeremy did say that his nasal passages were instantly cleared after this treatment, its still something Im not too anxious to try anytime soon. Still, its better than Mitras proposed cure for the same ailment, which she forced upon our other suitemate Gabe 08, also suffering from terrible congestion.

Bare Knuckles Boxing

For much of the 19th century boxing was not considered a legitimate sport in America. It was generally outlawed as a notorious crime, and boxing matches would be raided by the police and the participants arrested. Despite the official prohibitions against boxing matches, boxers often met in celebrated fights which drew large crowds and were openly reported in newspapers. And in the era before padded gloves became standard gear, the action in the bare-knuckle era was particularly brutal. Did You Know? Boxing was generally illegal in 19th century America, with fights held in secret locations.Bare-knuckle bouts were brutal, and could last for hours.Fighters could become famous, and some, peculiarly, picked up a political following.One bare-knuckles champion went on to serve in Congress. Despite the fame of some boxers, matches often tended to be scraps organized by neighborhood political bosses or outright gangsters. The fights could go on for hours, with opponents battering away at each other until one collapsed or was beaten insensible. While the contests involved punching, the action bore scant resemblance to modern boxing matches. The nature of the fighters was also different. As boxing was generally outlawed, there were no professional fighters. The pugilists tended to be otherwise employed. For instance, one noted bare-knuckles fighter in New York City, Bill Poole, was by trade a butcher, and was widely known as Bill the Butcher. (His life was very loosely adapted and portrayed in the Martin Scorsese film Gangs of New York.) Despite the notoriety and underground nature of bare knuckles fighting, some participants not only became famous, but were widely respected. Bill the Butcher, became a leader of the Know-Nothing Party in New York City before being assassinated. His funeral drew thousands of mourners, and was the largest public gathering in New York City until Abraham Lincolns funeral in April 1865. A perennial rival of Poole, John Morrissey, regularly found work as an election-day enforcer for New York City political factions. With what he earned boxing he opened saloons and gambling joints. His pugilistic reputation helped Morrissey to eventually be elected to Congress, representing a New York City district. John Morrissey during his bare knuckles boxing career. Library of Congress While serving on Capitol Hill, Morrissey became a popular figure. Visitors to Congress often wanted to meet the man known as Old Smoke, a nickname he picked up in a saloon fight when an opponent backed him up against a coal stove and set his clothes on fire. Morrissey, incidentally, proved he had enormous tolerance for pain when he won that particular fight. Later in the 19th century, when the boxer John L. Sullivan became popular, boxing became somewhat more legitimate. Still, the air of menace continued to surround boxing, and  major bouts were often held in peculiarly remote locations designed to skirt local laws. And publications like the Police Gazette, which focused on boxing events, seemed happy to make boxing seem shady. The London Rules Most boxing matches of the early 1800s were conducted under the London Rules, which were based on a set of rules laid down by an English boxer, Jack Broughton, in 1743. The basic premise of the Broughton Rules, and the subsequent London Prize Ring Rules, were that a round in a fight would last until a man went down. And there was a 30-second rest period between each round. Following the rest period, each fighter would have eight seconds to come to what was known as the scratch line in the middle of the ring. The fight would end when one of the fighters could not stand, or could not make it to the scratch line. Theoretically there was no limit to the number of rounds fought, so fights could go on for dozens of rounds. And because the fighters punched with bare hands, they could break their own hands by attempting knock-out punches to their opponents heads. So matches tended to be long battles of endurance. Marquess of Queensberry Rules A change in rules occurred in the 1860s in England. An aristocrat and sportsman, John Douglas, who held the title of the Marquess of Queensberry, developed a set of rules based on the use of padded gloves. The new rules came into use in the United States in the 1880s.

Determination Of Solubility Of Polar Solutes - 1074 Words

Introduction In the extraction of certain compounds, solubility plays an important factor in how the procedure is performed. The identification of which solvent is best for the substance is crucial. The relation of polarity between the solute and solvent is determined at a molecular level. The relationship between the solvent and solute needed shared similar characteristics. Polar solutes are dissolved in polar solvents while nonpolar solutes are dissolved in nonpolar solvents. From a chemical level, molecules are attracted to other similar molecules closest to their properties. Polar molecules are only attracted to polar molecules in both the solute and the solvent, and the same goes for nonpolar molecules. This explanation†¦show more content†¦For example, in some alkaline solvents acidic products will become deprotonated, keeping them soluble in water. The target compound can then be extracted as a free base. The extract results then are isolated by the addition of two immiscible sol vents. Two solvents that cannot be mixed are used so that there is a clear separation between the aqueous and organic layer. The component needed for extraction is contained in the organic layer. This is because the compound is more soluble to the composition of the organic layer. Multiple extractions with smaller volumes are more efficient than a single extraction at a one large volume. When an organic solvent is used to extract a compound from the aqueous solvent, smaller volumes will result in a better extraction. The success upon the collection of the crude material is depended on how well the water is absorbed by the anhydrous drying material. The presence of the drying material correlates with the vapor pressure of the other compound. When the vapor pressure is low, there is a smaller amount of moisture in the gas produced. Once the anhydrous material is added and clumping was avoided, the evaporation of the added organic substance can proceed. The final material collected can be physically identified by the final color of the precipitate. A greenish- white precipitate is most likely identified to be pure, and a brownish color indicates that the collected material is wet, and notShow MoreRelatedThin Layer Chromatography Lab Report1791 Words   |  8 Pagesmolecules, and determines the identity of a mixture of compounds. STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS [1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69  ºCMelting Point: -95 ºCDensity: 0.659 g/mL at 25 ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous Information: May cause irritation to the eyes, skin and respiratory system. May cause drowsiness and/or dizziness. Suspected of damaging fertility or unborn child. May cause damage to organsRead MoreQuantitative Analysis Cheat Sheet6271 Words   |  26 Pagessubstance: number of some specified elementary entity: mole (quantity: amount) unit: mole One serving of milk contains 195 mg PHOSPHORUS (matrix ïÆ'   value ïÆ'   unit ïÆ'   specie/analyte) Matrix: where the specie is being determined Concentration: how much solute is contained in a given mass or volume Molarity: mol/L * Molality: mol/kg *weight percent: mass of substance per mass solution (g/g % or wt%) *volume percent: Vol % * mole fraction: amount of substance in the total amount of solution (unitless) Read MoreRecrystallization And Identification Of Unknown1796 Words   |  8 Pagesdissolves the solute, the solute molecules-both the pure parts and the impurities- float among the solvent molecules. After the solution has cooled, the solvent is not able to hold any of the solute molecules any more, and they begin to form solid crystals at the surface. Each solute molecule will approach the crystal and rest on it; if its geometry fits that of the crystal, it is more likely to remain on the crystal than to go back into the solvent. Thus, the crystals only contain the solute moleculesRead MoreDetermination of Unknown Liquid (2-Butanol)2586 Words   |  11 Pagesmethods to deductively identify compounds (1) . The determination of unknown #6 (2-butanol) was identified by a ser ies of test; first taking the boiling point (94-96 C), performing a solubility test, Beilstien test, Ignition test, and the appropriate functional group tests. An infrared spectrum and an NMR spectrum were then obtained and confirmation of the compound was proved. 2-butanol (sec-butyl alcohol) Introduction: The determination of unknowns is an important skill to organic chemistRead MoreExtraction and Characterization of Proteins3660 Words   |  15 Pagesobtained and stored in crystalline form. (Boyer, 2000) The solubility behavior of proteins is largely affected by the ionic strength. In general, this behavior is described by a curve like the one below. As the ionic strength increases, protein solubility increases at first. This is refered to as â€Å"salting in†. Although, beyond a certain point, the solubility begins to decrease and this is known as â€Å"salting out†. (Boyer, 2000) Figure 1. Solubility of proteins If the ionic strength is low, the activityRead MoreSynthesis of Salicylic Acid and Potentiometric Determination of Its Purity and Dissociation Constant4209 Words   |  17 PagesSynthesis of Salicylic Acid and Potentiometric Determination of its Purity and Dissociation Constant ------------------------------------------------- Abstract The purpose of the study is to synthesize salicylic acid from the ester, methyl salicylate, and determine the acid’s dissociation constant and purity. The ester was converted to salicylic acid by base hydrolysis. The products were refluxed and recrystallized, to ensure maximum purity, and filtered, dried, and weighed. The melting point

Permanent Establishment Free Essays

The concept of permanent establishment (PE) in tax jurisdiction is changing as a result of digital innovations in international business. For a country like Nigeria, permanent establishment allows the country to tax the income or profit of foreign companies with a fixed place or physical presence in the country for 183 days or 12 months. But digital businesses do not need a fixed place or maintain a physical presence in the country before they can make a profit. We will write a custom essay sample on Permanent Establishment or any similar topic only for you Order Now They do not necessarily need to have an office, a factory, or a workshop in Nigeria before generating a stable income from the country. Examples of digital businesses that make income from Nigeria include GoDaddy.Com, Amazon.Com, Ebay.Com, etc. These companies do not have a fixed place of operation in Nigeria but generate income through digital presence in the country. This puts to test the concept of a permanent establishment in Nigerian tax jurisdiction. To understand the nature of permanent establishment in a digital economy, there is a need to investigate taxation jurisdiction on digital business from the angle of a developing country such as Nigeria. This study will be guided by the following objectives:Examine the principle of permanent establishment as expounded in the United Nations (UN) and Organisation for Economic Co-operation and Development (OECD) Model;Discuss the impact of digital business on the concept of a permanent establishment? Explore the possibility for a source state, such as Nigeria, to tax incomes generated by web platforms (i.e. Google or Facebook);Identify and discuss the challenges faced by the Nigerian government in getting taxes from companies who operate in the digital economy;Use the new definition of a permanent establishment in Italy to analyze tax jurisdiction on digital business in Nigeria. This study will adopt qualitative research method of legal research, analyze the concept of permanent establishment as a framework for tax jurisdiction on digital business in Nigeria. Primary documents such as the Nigerian tax laws (including the Avoidance of Double Taxation Agreement), the Italian tax laws (Conventions to Avoid Double Taxation), the UN Model Double Taxation Convention, the OECD Model and other documents that are relevant will be explored in the study. Secondary source materials covering monographs, journal articles, magazines, books, movies, textbooks, long essays, dissertations, and theses will equally be explored to analyze tax jurisdiction in digital business in Nigeria. Content analysis will be used to study legal decisions relating to the permanent establishment and digital business in Nigeria. How to cite Permanent Establishment, Papers

Hawaii Weddings free essay sample

Wedding Style in Hawaii Like most of the rest of the world weddings, in Hawaii symbolizes tradition and cultural elements in a colorful and beautiful place intended to bring fortune, luck and happiness to couples who are going to be for the rest of their lives together. Hawaiian brides, traditionally wear a Hawaiian dress called holoku. A holoku is a wedding dress with flowers and in her hair as well. Men usually wear a white shirt, white pants and a colorful sash. Casual foot wear is permitted, unless if the wedding is inside a house, footwear may be avoided. Another Hawaiian symbol for wedding eremony is the leis. Leis are a traditional flower garlands worn around the neck. Two main leis made of fragrant flowers and green leaves are especially made for the groom and bride. These main leis represent the peoples relationship with nature as a traditional aspect. The brides lei have white Jasmine and the grooms lei have Lima. We will write a custom essay sample on Hawaii Weddings or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Also, both the mother-in-laws wear especially made leis which have an abundance of jasmine. The weddings are announced by three blows ofa conch shell in a consecutive manner. This conch shell represents the summoning of God to support the ceremony. Fireworks also are part of the weddings symbolizing the pushing of evil away. The performance of dance called pandango is required, its also performed in many weddings and its often called the money dance, encircling the couples with a strong of taped money. Cultures around the world has affected Hawaiian weddings as well, as one of the influences is the wedding cake. Hawaiian wedding cakes are usually made of wheat flour, symbolizing the beginning of a new relationship together. This relationship is further strengthened by sharing the knife for cake cutting and presenting the first slice to each other.

Analysis of Feathers, a Short Story by Raymond Carver

Analysis of 'Feathers,' a Short Story by Raymond Carver American poet and author Raymond Carver (1938 - 1988) is one of those rare writers who is known, like  Alice Munro, primarily for his work in the short story form. Due to his economical use of language, Carver is often associated with a literary movement known as minimalism, but he himself objected to the term. In a 1983 interview, he said, Theres something about minimalist that smacks of smallness of vision and execution that I dont like. Feathers is the opening story of Carvers 1983 collection, Cathedral, in which he began to move away from the minimalist style. Plot of Feathers SPOILER ALERT: If you dont want to know what happens in the story, dont read this section. The narrator, Jack, and his wife, Fran, are invited to dinner at the home of Bud and Olla. Bud and Jack are friends from work, but no one else in the story has met before. Fran is not enthusiastic about going.   Bud and Olla live in the country and have a baby and a pet peacock. Jack, Fran, and Bud watch television while Olla prepares dinner and occasionally tends to the baby, who is fussing in another room. Fran notices a plaster cast of very crooked teeth sitting on top of the television. When Olla enters the room, she explains that Bud paid for her to have braces, so she keeps the cast to remind me how much I owe Bud. During dinner, the baby begins fussing again, so Olla brings him to the table. He is shockingly  ugly, but  Fran holds him  and delights in him in spite of his appearance. The peacock is permitted inside the house and plays gently with the baby. Later that night, Jack and Fran conceive a child even though they had not previously wanted children. As the years pass, their marriage sours and their child demonstrates a conniving streak. Fran blames their problems on Bud and Olla even though she saw them only on that one night. Wishes Wishes play a prominent role in the story. Jack explains that he and Fran regularly wished out loud for things we didnt have, like a new car or the chance to spend a couple of weeks in Canada. They dont wish for children because they dont want children. It is clear that the wishes arent serious. Jack acknowledges as much when he describes approaching Bud and Ollas house: I said, I wish we had us a place out here. It was just an idle thought, another wish that wouldnt amount to anything. In contrast, Olla is a character who has actually made her wishes come true. Or rather, she and Bud together have made her wishes come true. She tells Jack and Fran: I always dreamed of having me a peacock. Since I was a girl and found a picture of one in a magazine. The peacock is loud and exotic. Neither Jack nor Fran has ever seen one before, and it is much more dramatic than any of the idle wishes theyve been making. Yet Olla, an unassuming woman with an ugly baby and teeth that needed straightening, has made it a part of her life. Blame Though Jack would place the date later, Fran believes their marriage began to deteriorate precisely on the night they had dinner at Bud and Ollas, and she blames Bud and Olla for it. Jack explains: Goddamn those people and their ugly baby, Fran will say, for no apparent reason, while were watching TV late at night. Carver never makes it clear exactly what Fran blames them for, nor does he make it clear exactly why the dinner gathering inspires Jack and Fran to have a baby. Perhaps its because Bud and Olla seem so happy with their strange, squawking-peacock, ugly-baby lives. Fran and Jack dont think they want the particulars - a child, a house in the country, and certainly not a peacock - yet perhaps they find they do want the contentedness that Bud and Olla seem to have. And in some ways, Olla does give the impression that her happiness is a direct result of the particulars of her situation. Olla compliments Fran on her naturally straight teeth while she herself had required braces - and Buds devotion - to fix her crooked smile. At one point, Olla says, You wait until you get our own baby, Fran. Youll see. And as Fran and Jack are leaving, Olla even hands Fran some peacock feathers to take home. Gratitude But  Fran seems to be missing one fundamental element that Olla has: gratitude. When Olla explains how grateful she is to Bud for straightening her teeth (and, more generally, giving her a better life), Fran doesnt hear her because she is picking through the can of nuts, helping herself to the cashews. The impression is that Fran is self-centered, so focused on her own needs that she cant even hear someone elses expression of gratitude. Similarly, it seems symbolic that when Bud says grace, Olla is the only one who says amen. Where Happiness Comes From Jack does note one wish that came true: What I wished for was that Id never forget or otherwise let go of that evening. Thats one wish of mine that came true. And it was bad luck for me that it did. The evening seemed very special to him, and it left him feeling good about almost everything in my life. But he and Fran may have miscalculated where that good feeling was coming from, thinking it came from having things, like a baby, rather than feeling things, like love and appreciation.

Convert Frequency to Wavelength Worked Example Problem

Convert Frequency to Wavelength Worked Example Problem This example problem demonstrates how to find the wavelength of light from the frequency. Frequency vs Wavelength The wavelength of light (or other waves) is the distance between subsequent crests, valleys, or other fixed points. The frequency is the number of waves that pass a given point in one second. Frequency and wavelength are related terms used to describe electromagnetic radiation or light. One simple equation is used to convert between them: frequency x wavelength speed of light ÃŽ » v c, when ÃŽ » is wavelength, v is frequency, and c is the speed of light so wavelength speed of light / frequency frequency speed of light / wavelength The higher the frequency, the shorter the wavelength. The usual unit for frequency is Hertz or Hz, which is 1 oscillation per second. Wavelength is reported in units of distance, which often ranges from nanometers to meters. Conversions between frequency and wavelength most often involve wavelength in meters because thats how most people remember the speed of light in a vacuum. Key Takeaways: Frequency to Wavelength Conversion Frequency is how many waves pass a defined point per second. Wavelength is the distance between successive peaks or valleys of a wave.Frequency multiplied by wavelength equals the speed of light. So, if you know either the frequency or the wavelength you can calculate the other value. Frequency To Wavelength Conversion Problem The Aurora Borealis is a night display in the Northern latitudes caused by ionizing radiation interacting with the Earths magnetic field and the upper atmosphere. The distinctive green color is caused by the interaction of the radiation with oxygen and has a frequency of 5.38 x 1014 Hz. What is the wavelength of this light?Solution:The speed of light, c, is equal to the product of the wavelength, lamda;, and the frequency, ÃŽ ½.ThereforeÃŽ » c/ÃŽ ½ÃŽ » 3 x 108 m/sec/(5.38 x 1014 Hz)ÃŽ » 5.576 x 10-7 m1 nm 10-9 mÃŽ » 557.6 nmAnswer:The wavelength of the green light is 5.576 x 10-7 m or 557.6 nm.

Asset Classification Policies and Procedures Essay

Asset Classification Policies and Procedures - Essay Example Asset Classification Policies and Procedures For information to be handled properly, employees are required to have a working knowledge of the categorization of information into the three provided categories. Employees should be able to categorize the information before forwarding it further. If at some point, employees are confused about properly categorizing the information, the proper course of action is to classify it as confidential while an appropriate supervisor would later review and properly classify. Public Data- As the name suggests, such data is usually open to the public and is easily available. Disclosure of such data does not put the firm in any kind of risk; however certain controls are required to be enforced on such data to prevent modification or destruction of the data by unauthorized parties; Sensitive Data- Data is classified as sensitive data when disclosures of such information publically can result in potential risk for the organization or its people. Such information may be provided to others on a discretionary basis and under the supervision of the data owner. Confidential Data – Confidential data is the most sensitive data within the organization and unauthorized disclosure of such information can result in significant risk for the firm. The highest level of security and control are applied on such information. The System Impact level will determine the impact of activities on the system on a scale of one to five with five being the most crucial impact and one having the least crucial impact.

TOURIST ATTRACTION SITE VISIT Essay Example | Topics and Well Written Essays - 500 words

TOURIST ATTRACTION SITE VISIT - Essay Example These hotels provide amazing facilities to tourists. I asked organizing committee about procedures for purchasing tickets for soccer season. They replied, online tickets for complete season can be purchased at cheap rates and it is also possible to select the place of own choice for watching the matches in stadium. I asked organizers that what all facilities are available at the stadium for watching soccer competitions. They replied, they have introduced great facilities like, bathrooms, food, cheerleaders, sound effects and screens for adding all sorts of fun in the events. Who all are tough teams in the event? Mayor said, â€Å"Houston Dynamo† and â€Å"Dallas† are the best teams of the event. I asked how many stadiums are available for hosting the event. Mayor counted four stadiums including a newly constructed state of the art stadium. What is tailgating in Houston? Mayor said that you have snacks and hotdogs before start of the match is tailgating. What are the other tourist destinations in Houston? Mayor replied, space Centre and downtown aquarium are worth visiting places. Organizing committee told me to visit Orange Avenue as well. This avenue provides free souvenirs, food, shirts and a band plays there to entertain people. I am enjoying the opening games 2014 more than my expectations. It is so fascinating and colourful for people of any place in the world. I heard about the Houston and soccer events from my friend and then visited online sources. After coming here, I am not ready to leave this place now. Event is so organized that tourists do not feel any problem in their activities. There is one problem that spectators cannot buy single ticket for a match and they have to buy for complete season. Organizers need to look into this matter. Moreover, stadiums with more capacity should be constructed for accommodating huge crowds. Dynamo started the season against â€Å"New England Revolution† on 8 Mar 2014. There is a long

Cahi-DRB and DQB1 Alleles in Sirohi Goat

Cahi-DRB and DQB1 Alleles in Sirohi Goat Genetic diversity of DRB and DQB genes of caprine MHC class II in Sirohi goat G. R. Gowane, Najif Akram, S.S. Misra, Ved Prakash and Arun Kumar Running Head: CahiDRB and DQB1 alleles in Sirohi goat ABSTRACT Objective of the study was to assess the genetic diversity of the Sirohi goat for DRB and DQB1 loci and to study their association with antibody response induced by the Peste des petits ruminants (PPR) vaccine. A total of 360 Sirohi kids were studied using Single Stranded Confirmation Polymorphism (SSCP) followed by Sequence Based Typing (SBT)-PCR for DRB and DQB1 diversity. C-ELISA was used to assess immune response post PPR vaccination. Study revealed rich diversity of MHC region. A total of 18 DRB and 15 DQB1 alleles were obtained. Allele DRB*0104 and allele DQB1*0101 were most common. All the alleles reported are new. Study revealed variability in DRB and DQB1 region not only at nucleotide but also at amino acid level with high Wu-Kabat index. A total of 16 out of 89 amino acid residue sites had more than 3 amino acid substitutions in DRB. Similarly, 19 out of 86 residue sites in DQB1 had more than 3 amino acid substitutions. Positive evolutionary selection was evident in Sirohi for MHC region. Non-significant association of DRB and DQB1 genotypes with PPRV vaccine response revealed complexity of the phenotype and importance of other factors for vaccine response. Rich diversity of DRB and DQB1 gene reflects the fitness of the population and importance of this locus for future selection programs. Keywords: Cahi-DRB, Cahi-DQB1, Major histocompatibility complex, Vaccine response 1. Introduction Major Histocompatibility Complex (MHC) of goats is polymorphic. A few of the genes such as Caprine Leukocyte Antigen (Cahi)-DRB and Cahi-DQB1 from this complex are recently being investigated for their polymorphism and further potential association with important diseases of goat. The class II antigens encoded by MHC class II genes bind to processed peptides from extracellular antigens and present them to epitope specific CD4+ T lymphocytes. Cahi-DRB exon 2 is polymorphic so is Cahi-DQB1, due to their importance in antigen binding groove formation and evolutionary importance in antigen capture and presentation. Peptide binding site (PBS) in goat is partly coded by DRB and DQB gene. This PBS has several pockets which are highly variable and accommodate the side chains of the bound peptide. A non-synonymous change in the nucleotide sequence of the MHC DRB or DQB1 gene can substantially substitute the coding amino acid and ultimately bring conformational change in the binding groove so as to affect the efficiency of the protein to present the antigen efficiently for further processing. Several reports exists which link the variability in DRB alleles in cattle, sheep and other mammals to resistance or susceptibility to diseases. Herrmann-Hoesing et al. [1] reported that Ovar-DRB1 alleles contribute as a host genetic factor that control provirus level in sheep. Significant association of DRB1 alleles with susceptibility and resistance to Ovine pulmonary adenocarcinoma (OPA) was reported by Larruskain et al. [2] in sheep. However as far as studies on goat are concerned, there are very few caprine DRB and DQB1 sequences in Gene Bank. Similarly, there is a scarcity of research database for allelic association of DRB and DQB alleles with disease resistance or susceptibility in goat. It is for no surprise that even the IPD-MHC database has no space dedicated for goat MHC. Amills et al. [3] assessed the genetic variability in DRB of goat. This was followed by a few report s [4-8] to characterize DRB locus (exon 2 of DRB) in goat. Amills et al. (2004) also characterized DQB1 locus in goat, however not much work [9] has been carried out since then for its genetic polymorphism. Genetic variability in response to vaccination is likely to become an even more significant factor in designing ideal vaccines [10]. The genes identified might also be important for disease resistance traits, and could potentially provide the tools to select good responders opening the doors for potential implications in future selection programme [11,12]. The Peste des petits ruminant (PPR) being the plague of small ruminants pose heavy threat to the rural economy of India. It is caused by a PPR virus (PPRV) of the genus Morbillivirus within the family Paramyxoviridae. India constitutes a great diversity of small ruminants with 135.17 million goat and 65.07 million sheep (19th Livestock census) [13]. In PPRV endemic regions including India, control measures involve regular vaccination with live attenuated PPR virus vaccine of lineage IV, which has high antigenic stability and induce long term immune response [14]. Currently, three live attenuated PPR vaccines (Sungri/96, Arasur/87 and CBE/97 stains) are available in India for prevention of this disease, of which, Sungri/96, developed by ICAR-Indian Veterinary Research Institute (IVRI), Mukteswar has undergone extensive field trial [15-17]. It is possible that the vaccine induced protection across individuals is not homogenous, wherein, vaccine gives a complete protection for a proportion of individuals while rest acquire only incomplete (leaky) protection of varying magnitude [18]. Variable vaccine response in the population has been reported for several diseases in humans as well as animals [19-27]. Role of host genetics and other non-genetic factors in variation to vaccine response especially for PPR vaccine has not been studied till today in details. The importance of host genetics in vaccine response studies is important as genetic variability may influence vaccine response and hence confound vaccine efficacy studies. Objective of the present study is to decipher the Cahi-DRB and Cahi-DQB1 polymorphisms in detail using sequence based typing polymerase chain reaction (SBT-PCR) and to associate the variation obtained with PPR vaccine elicited immune response in Sirohi goat kids maintained at the farm condition in semi-arid region of India. 2. Materials and Methods 2.1 Animals The study population was a flock of purebred Sirohi goats. The flock was located at ICAR-Central Sheep Wool Research Institute, Avikanagar in the semi-arid region of Rajasthan, India at 75025â‚ ¬Ã‚ ²E, 26018â‚ ¬Ã‚ ²N, at an altitude of 320 m above mean sea level. The data for the experiment involved 360 Sirohi goat kids. All the animals under the study belonged to same age group, i.e. weaner with mean age at vaccination 142.43 days (SD = 14.67). All the animals in this flock were kept under semi-intensive management system.   Concentrate mixture was offered ad libitum to suckling kids from 15 days of age till weaning (90 days). After 3 weeks of age till weaning, kids were sent for grazing for 3 h each in morning and evening, but not along with their dams. During the post-weaning period in addition to 8-10 h grazing and dry fodder supplementation, 300 g of concentrate mixture was provided in the evening hours after browsing. The grazing area consisted of forestland with natur al fodder trees like Khejri (Prosopis cineraria), Ardu (Ailanthus spp.), and Neem (Azadirecta indica). Bushes and surface vegetation including the improved pastures of Cenchrus ciliarisis are also available. Due to scarce grazing resources from March to June, the goats were supplemented with hay of Cenchrus, Cowpea, and Dolichos; pala leaves (Zizyphus) and fodder tree lopping. 2.2 Amplification and typing of DRB alleles Whole blood (1 ml) was collected aseptically from the jugular vein of lambs for DNA isolation (GenElute Blood Genomic DNA Kit, SIGMA) according to the manufacturers instructions. Exon 2 of the DRB gene was amplified from genomic DNA using the primers as suggested by Amills et al. [3], where DRB.1: 5-TATCCCGTCTCTGCAGCACATTTC-3 and DRB.2: 5-TCGCCGCTGCACACTGAAACTCTC-3 primers were used for amplifying 285 bp product. The reaction mixture of 50ÃŽÂ ¼l comprised of: 10X Taq Buffer (05ÃŽÂ ¼l), 25mM MgCl2 (03ÃŽÂ ¼l), 10mM dNTP (1ÃŽÂ ¼l), 20 pmol (1ÃŽÂ ¼l) of each primer, Taq DNA Polymerase (1IU), Template (1ÃŽÂ ¼l) and Nuclease Free Water (NFW) to make 50ÃŽÂ ¼l. The thermal profile was optimized for amplification of the DRB exon2 as follows: Initial denaturation (94 °C for 4 min), followed by 35 cycles (denaturation for 94 °C for 60 s, annealing at 66 °C for 60s and extension at 72 °C for 60s) and a final extension at 72 °C for 5 min. A single clear band of 285 bp on agarose gel (2%) was obtained. The amplified products were subjected to Single Stranded Confirmation Polymorphism (SSCP) for determination of the genotypic variation [28]. The samples were then grouped according to various genotypes as obtained on the SSCP gel. The representative samples were then again amplified using the PCR protocol as above and purified PCR products (GenElute„ ¢ Gel Extraction Kit, SIGMA) were sequenced by BigDye (Applied Biosystems, USA) sequencing reaction that exploits di-deoxy chain termination principle. The PCR-Sequence Based Typing (PCR-SBT) was used for further analysis. The homozygous sequences obtained were assigned an allelic name using nomenclature system as suggested by Ballingall and Tassi [29] to suit IPD MHC nomenclature system. The heterozygote samples were re-sequenced after cloning (InsTAclone PCR Cloning Kit, Thermo Fisher) to obtain one allele that was subsequently used to deduce another allele in heterozygous sample. Novel alleles were cloned, sequenced and confir med at least thrice. The amino acids at pocket positions were determined from the nucleotide sequences of the alleles using EditSeq software package V5.0 [30]. Alleles which were derived and not confirmed in SBT-PCR were not named. 2.3 Amplification and typing of DQB1 alleles Exon 2 of the DQB1 gene was amplified from genomic DNA using the primers as described by Amills et al. [31], where DQB-F: 5- CCC CGC AGA GGA TTT CGT G -3 and DQB-R: 5- ACC TCG CCG CTG CCA GGT -3 primers were used for amplifying 280 bp product having 8bp intron1, 270bp exon2 and 2bp intron2. The reaction mixture of 50ÃŽÂ ¼l comprised of: 10X Taq Buffer (05ÃŽÂ ¼l), 25mM MgCl2 (03ÃŽÂ ¼l), 10mM dNTP (1ÃŽÂ ¼l), 20 pmol (1ÃŽÂ ¼l) of each primer, Taq DNA Polymerase (1IU), Template (1ÃŽÂ ¼l) and Nuclease Free Water (NFW) to make 50ÃŽÂ ¼l. The thermal profile was optimized for amplification of the DQB exon2 as follows: Initial denaturation (94 °C for 4 min), followed by 35 cycles (denaturation for 94 °C for 45 s, annealing at 67 °C for 45s and extension at 72 °C for 45s) and a final extension at 72 °C for 5 min. A single clear band of 280 bp on agarose gel (2%) was obtained. The amplified products were subjected to Single Stranded Confirmation Polymorphism (SSCP ) for determination of the genotypic variation [28]. The samples were then grouped according to various genotypes as obtained on the SSCP gel. The PCR-SBT approach was used for analysis. Alleles were named as per requirements of the IPD-MHC database [29], derived alleles were not named. 2.4 PPR Vaccination, Sampling and ELISA for detection of antibody against PPRV vaccine As part of the scheduled vaccination program, the animals were vaccinated (1 ml subcutaneous) with freeze dried live attenuated PPR virus (Sungri 96 strain) vaccine with PPR virus titre †°Ã‚ ¥ 102.5 TCID50 (Raksha-PPR, Indian Immunologicals, India).   Whole blood was collected aseptically by jugular vein puncture from the kids at 28 days post vaccination (28DPV) for serum separation. Serum was collected and stored at ˆ’20- ¦C until testing. The ELISA for further analysis was done as described earlier [27]. 2.6 Statistical Analysis The allelic frequencies, genotypic frequencies, phylogenetic analysis and residue substitution was studied using Microsoft excel package of the MS office (2010) and EditSeq (DNA STAR) software. Phylogenetic analysis was performed using MEGA 4.0, neighbor joining method. To assess the effect of genotype on vaccine response (observed PI values), a General Linear Model (GLM) was used that included Cohort (2 levels), Sex (2 levels), age at vaccination (continuous) as fixed effects along with either DRB or DQB1 genotype. All the above analyses were performed using a statistical package SPSS [32]. 2.7 The dn/ds ratio and Wu Kabat variability index The frequencies of non-synonymous (dn) versus synonymous (ds) substitutions were calculated by the method of Yang and Nielsen [33] with the help of software PAML 4 [34]. The Wu Kabat variability index with respect to amino acids at peptide binding pockets was calculated using the formula given by Wu and Kabat [35]. Index =  Ã‚  Ã‚   The number of different amino acids occurring at a given position  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Frequency of the most common amino acid at the position Where, frequency of the common amino acid is obtained as number of times the most common amino acid occurs divided by the total number of protein examined. 3. Results and Discussion 3.1 Genetic variability for DRB Sirohi goat kids (N=360) were typed for DRB exon 2. A total of 18 new alleles were obtained after analysis in the population using SBT-PCR approach (Table 1). Out of the 18 alleles, 12 alleles were confirmed by cloning and sequencing, however 6 were derived using SBT-PCR. All 12 alleles were new and named as per the requirements of the Immuno-Polymorphism Database (IPD) following guidelines [29]. Alleles were Cahi-DRB*0701  Ã‚   (accession no. KX431913), Cahi-DRB*0104  Ã‚   (accession no. KX431914), Cahi-DRB*0402 (accession no. KX431915), Cahi-DRB*0102  Ã‚   (accession no. KX431916), Cahi-DRB*0202  Ã‚   (accession no. KX431917), Cahi-DRB*0501 (accession no. KX431918), Cahi-DRB*0401 (accession no. KX431919), Cahi-DRB*0103 (accession no. KX431920), Cahi-DRB*0203 (accession no. KX431921), Cahi-DRB*0101 (accession no. KX431922), Cahi-DRB*0201 (accession no. KX431923) and Cahi-DRB*0601 (accession no. KX431924). A total of 6 new alleles were derived using PCR-SBT approach, however not given names as per IPD-MHC nomenclature (N7, N11, N13, N16, N17, N18). Allele CahiDRB*0104 had highest frequency 29.72% followed by *0701 allele (22.64%), *0202 (13.89%) and *0102 (11.25%). In congruence with our finding, rich diversity of this region has been reported earlier in different goat breeds worldwide [3-8]. However, most of the studies were carried out using Restriction Fragment Length Polymorphism (RFLP) PCR, whereas, the current method of SSCP followed by SBT-PCR has more power to detect the genetic variability at DRB in goat. The ratio of non-synonymous (dN) to synonymous (dS) substitution for DRB gene in the Sirohi goat population was 3.24. This ratio was significantly greater than 1 indicating positive evolutionary selection for DRB gene in the present populations. However, the results are read with caution as the evidence is presumed and not absolute, due to lack of evidence for Capra species. It may be impossible to infer the selection pressure from the dN/dS measurement [36]. In another study, 11.1 ratio for dN/dS was recorded in Peptide Binding Region of 12 Chinese indigenous goats for DRB*02 sequences [6]. PBR being polymorphic, its importance is seen here. According to Simmons et al. [37], the long-term evolution, ancient and silent mutations also carried with translated mutations and became maintained in these regions. Pathogen-host interaction is complex, according to the Red Queen hypothesis [38], to be a part of this competition, diversity of MHC is important from hosts perspective. Plotting the phylogenetic tree for allelic relationship at nucleotide level revealed that the diversity was large (Fig 1). Clustering of the alleles revealed that some alleles tended to form closer clusters than others. Fig 2 reveals the amino acid variation between the alleles and it is seen that the population is polymorphic at coding region too, thus providing enough raw material for Sirohi goat population to tackle the pathogen variability. Study found that alleles DRB*0101, *0102, *0103 and *0104 had less than or equal to 4 codon change and hence clubbed together in one family. Derived allele *N18 also formed member of this group due to similarity of amino acid sequence. Similarly, alleles *0201, *0202, and *0203 had less than 4 amino acid differences. Alleles *0401 and *0402 had less than 4 amino acid differences, whereas, alleles *0501, *0601 and *0701 differed by more than 4 amino acid differences from each group. Predicted allele *N7 was related to *0701 due to similarity at amino acid level. Derived alleles *N11, *N13 and *N17 formed a group separate from others, similarly derived allele *N16 formed a different group. Phylogenetic analysis revealed that clustering based on nucleotide similarity and differences remained almost similar to clustering based on amino acid differences. 3.2 Genetic variability for DQB1 Sirohi goat kids (N=339) were typed for DQB1 exon 2. A total of 15 new alleles were obtained after analysis in the population using SBT-PCR approach (Table 1). Out of the 15 alleles, 13 alleles were confirmed by cloning and sequencing, however 2 were derived using SBT-PCR. All 13 alleles were new and named as per the requirements of the IPD [30]. Alleles were CahiDQB1*0101 (Accession number KX431925), CahiDQB1*0201 (Accession number KX431926), CahiDQB1*0301 (Accession number KX431927), CahiDQB1*0302 (Accession number KX431928), CahiDQB1*0103 (Accession number KX431929), CahiDQB1*0501 (Accession number KX431930), CahiDQB1*0104 (Accession number KX431931), CahiDQB1*0701 (Accession number KX431932), CahiDQB1*0801 (Accession number KX431933), CahiDQB1*0102 (Accession number KX431934), CahiDQB1*070101 (Accession number KX431935), CahiDQB1*0502 (Accession number KX431936) andCahiDQB1*0202 (Accession number KX431937). A total of 2 new alleles were derived using PCR-SBT approach, however not given names as per IPD-MHC nomenclature (*N2, *N3). Allele CahiDQB1*0101 had highest frequency 27.22% followed by *070101 allele (13.02%), *N2 (11.69%) and *0201 (11.54%). Very high genetic diversity for this region has also been reported earlier [3, 31]. Similar diversity is also observed in sheep and cattle DQB1 region, however for goat there are very few studies. This study is the first report for DQB1 diversity in any Indian goat breed. To study the evolutionary stability or instability of the DQB1 region in Sirohi goat, the ratio of non-synonymous (dN) to synonymous (dS) substitution for Sirohi goat has been studied. We found that the ratio was 1.08. Yakubu et al. [9] reported a ratio of 2.14 in Nigerian goat breeds.   Results reveal balancing selection in favour of variability at DQB1 in Sirohi goat. Phylogenetic analysis for alleles reported that the diversity at nucleotide level was large (Fig 1). There was a clustering of alleles for their nucleotide substitutions and thus clubbing in one or the other family. Fig 3 reveals the amino acid variation between the alleles and it is seen that the population is polymorphic at coding region. Alleles DQB1*0101, *0102, *0103 and *0104 were in one group as they had less than 4 amino acid changes. Similarly, alleles *0201, *0202, and *0203 had less than 4 amino acid differences. Alleles *0201 and *0202 formed another family, alleles *0301 and *0302 formed separate family, and alleles 0501 and 0502 were clubbed together. It was seen that derived alleles N3 had similarity at amino acid level with allele *0201, indicative of synonymous substitution at nucleotide level.   Alleles *0701 and 070101 were in one family and they did not have a single amino acid substitution. However, they had synonymous differences at nucleotide level that resul ted in the no change at peptide level. Derived allele *N2 was related with *N3, however placed in separate group due to differences at amino acid level. 3.3 Association of DRB and DQB1 genes with PPRV vaccine elicited immune response Results of C-ELISA on sera samples at 28DPV revealed mean PI value of 69.99 ±0.42 (Min 13.32, max 91.60) with minimum PI 35.12 and maximum PI 98.82.  Ã‚   Average   age   at vaccination   was   142.43    ±Ã‚   14.67   days   with   minimum   age   93 days   and maximum   age   164   days.   Variability in the vaccine response was evident in the lambs.   Frequency distribution of Ovar-DRB and DQB1 alleles revealed rich diversity amongst Sirohi goat. A total of 16 DRB genotypes and 16 DQB1 genotypes were observed to be present in the population of Sirohi goat flock. For association analysis, genotypes with >5 occurrences in the population (11 genotypes in DRB and 12 genotypes in DQB1) were only used to avoid biased estimates. Genotypic association analysis was carried out to assess the effect of genotype (Table 2) along with other environmental factors on vaccine response in Sirohi goat sheep. In the DRB group (N=299), Genotype I(DRB*0104-*0104) had highest frequency (30.10%) followed by genotype A(DRB*0701-*0701) 22.07% and genotype M(DRB*0202-*0202) 13.38%. In the DQB1 group (N=298), highest frequency was obtained for genotype E(DQB1*0801-*0801) 20.13%, followed by genotype J(DQB1*0301-*0101) 14.43% and genotype G(DQB1*0502-*0502) 11.41%. In the model that studied the effect of DRB genotype along with other environmental factors such as cohort, sex of the animal and age group, on vaccine response, explained 63.6% variation (R2=0.636). The genotypic association study revealed non-significant (P = 0.606) effect of genotype on 28DPV PI value, whereas significant effect of cohort and age at vaccination. However, ranking of genotypes revealed that the genotype L(DRB*0102-*0102) gave highest response for PPRV vaccination at 28th day (Table 2) followed by genotype J(DRB*0402-*0402) and A(DRB*0701-*0701).   Lowest response was obtained for the genotype E(DRB*0201-*0201) preceded by D(DRB*0101-*N13) and I(DRB*0104-*0104). It was noteworthy that alleles in high ranking genotypes were exclusive to low ranking genotypes. Effect of genotype was non-significant on the vaccine response, however, the trend was visible with increasing rank and declining mean PI for 28DPV (Table 2). The variability within DRB region of Sirohi goat population was calculated using the Wu-Kabat Variability Index (Table 3). The ability of a pocket to anchor a peptide is due to the electrostatic charges of the pocket region and electrostatic charges of the peptide [39]. Out of several amino acid positions in DRB, a total of 16 different amino acid positions were polymorphic with three or more than 3 amino acid differences (residue 6, 21, 32, 35, 37, 52, 61, 62, 65, 66, 68, 69, 72, 73, 76 and 81). The region revealed Wu-Kabat index varying from 2.20 to 6.95. Highest index was observed at residue 6 (6.95%), followed by ÃŽÂ ²65 (6.41%) and ÃŽÂ ²73 (5.94%).   Present results corroborates with the earlier observations in sheep breeds [41, 42], where positive selection at important residues in DRB1 amino acid sequence was observed. In DQB1 group, again the inclusive model could explain 62% of the total variation in the 28DPV vaccine response trait (R2=0.62). The model included sex, age and cohort of the animal along with the DQB1 genotype. The effect of genotype was non-significant (P = 0.868), however, the effect of cohort and age at vaccination were highly significant (PDQB1*0104-*0701) gave highest response for PPRV vaccination at 28th day (Table 2) followed by Genotype E(DQB1*0801-*0801) and I(DQB1*0201-*0201). Lowest response was obtained for the genotype D(DQB1*0101-*N3) preceded by A(DQB1*0101-*0101) and then by F(DQB1*070101-*070101). Alleles in low ranking genotypes and high ranking genotypes were exclusive to each other and hence represent the allelic substitution as an effect for change in the vaccine response. The variability within DQB1 region of Sirohi goat population was calculated using the Wu-Kabat Variability Index (Table 4).Our result suggest a lot of interesting sites in the amino acid structure of the DQB1, where substitution has taken place. The Wu-Kabat index reveal variability starting from 2.67 at ÃŽÂ ²29, ÃŽÂ ²60 to 7.19 at ÃŽÂ ²81. A total of 19 residues in the translated sequence of DQB1 were found to be polymorphic with at least three amino acid substitutions. Similar results were reported by Amills et al. (2004), where many amino acid residues within and outside the pockets were found to be polymorphic in nature. In present study, although a significant association of these substitutions with vaccine response is not observed, but variability of the region is well explored. Many factors influence the vaccine response as a trait in mammals. Role of environmental factors as well as other MHC and non-MHC genes is important, however apart from that the nature of the responding variable is also one of the most important criteria to look for in such analysis. PPR vaccine is a strong antigen and its invasion produces a cascade of reactions responsible for antibody production. In our earlier study [27], 94.92% Sirohi kids were observed to be protected with a single dose of PPRV vaccine. Therefore in spite of having variability within the protected category, the differences between the animals is not much and hence association of minor change in the phenotype vis a vis genotype is not visible.   There are several studies which revealed the effect of QTLs and non-genetic factors in detail showing the role of non-MHC genes and environmental influences on vaccine response [12,26,27,42,]. In goat, only one study [8] could show significant association of DRB gene p olymorphism obtained by PCR-RFLP with Johnes disease. Apart from this there are no studies which reveal association of MHC genotypes with disease resistance or susceptibility in goat. 4. Conclusion The genetic variability of DRB and DQB1 gene in Sirohi goat revealed a very rich diversity of this locus with positive evolutionary trend. Our study provide first description of the evidence of such a strong diversity of MHC in Indian goat breed for DRB and DQB region. Due to complex nature of the phenotype, i.e. vaccine response, and good response to the antigen used, association with studied loci was not observed. Apart from this several factors apart from MHC also affected the outcome of the response. Observed variability within the DRB and DQB1 loci reveals potential of the breed for combating several antigenic attacks and hence importance of the studied region in antigen capture and presentation to T cells. Acknowledgements Authors duly acknowledge Department of Biotechnology (GOI) for project grant to carry out the desired work. Authors are thankful to the Director ICAR-CSWRI for providing facilities for carrying out the work. Authors are also thankful to AICRP on Goat for funding the project on Sirohi goat at ICAR-CSWRI Avikanagar. Conflict of Interest Statement: The authors declare that they have no conflict of interest. References Herrmann-Hoesing LM, White SN, Mousel MR, Lewis GS, Knowles DP (2008) Ovine progressive pneumonia provirus levels associate with breed and Ovar DRB1. Immunogenet 60:749-758 Larruskain A, Minguijà ³n E, Garcà ­a-Etxebarria K, Moreno B, Arostegui I, Juste RA, Jugo BM (2010) MHC class II DRB1 gene polymorphism in the pathogenesis of Maedi-Visna and pulmonary adenocarcinoma viral diseases in sheep. Immunogenet 62:75-83 Amills M, Francino O (1995) Nested PCR allows the characterization of Taq I and Pst I RFLPs in the second exon of the Caprine MHC class II DRB gene. Vet Immunol Immunopathol 48:313-321 Amills M, Francino O (1996) A PCR-RFLP typing method for the Caprine MHC class II DRB gene. Vet Immunol Immunopathol 55:255-260 Dongxiao S, Yuan Z (2004) Polymorphisms of the second exon of MHC-DRB gene in Chinese local sheep and goat. Biochem Genet 42(9-10):385-390 Ahmed S, Othman OE (2006) The characterization of Hae III patterns in the second exon of the buffalo MHC class II DRB gene. Biotechnol J 5(4):514-516 Zhao Y, Zhao E, Zhang N, Duan C (2011) Mitochondrial DNA diversity, origin, and phylogenic relationships of three Chinese large-fat-tailed sheep breeds. Trop Anim Health Prod 43:1405-1410 Singh PK, Singh SV, Singh MK, Saxena VK, Singh AV, Sohal JS (2012) Genetic Analysis of MHC Class II DRB gene in an endangered Jamunapari breed of goats. Indian J Biotechnol 11(2):220-223 Yakubu A, Salako AE, De Donato M, Takeet MI, Peters SO, Adefenwa MA, Okpeku M, Wheto M, Agaviezor BO, Sanni TM, Ajayi OO, Onasanya GO, Ekundayo OJ, Ilori BM, Amusan SA, Imumorin IG (2013) Genetic Diversity in Exon 2 at the Major Histocompatibility Complex DQB1 Locus in Nigerian Indigenous Goats. Biochem Genet 51:954-966 Glass EJ, Baxter R, Leach RJ, Jann OC (2011) Genes controlling vaccine responses and disease resistance to respiratory viral pathogens in cattle. Vet Immunol Immunopathol 148(1-2):90-99 Wilkie BN, Mallard BA (1999) Selection for high immune response: an alternative approach to animal health maintenance. Vet Immunol Immunopathol 72:231-235 Gowane GR, Sharma AK, Sankar M, Narayanan K, Das B, Subramaniam S, Pattnaik B (2013b) Association of BoLA DRB alleles with variability in immune response among the crossbred cattle vaccinated for foot-and-mouth disease (FMD).Res Vet Sci 95:156-163 19th Livestock census, http://dahd.nic.in/sites/default/files/19%20th%20Livestock%20%202012. pdf, 2012 (accessed 19.07.2016). Venkataramanan R, Bandyopadhyay SK, Oberoi MS (2005) Present status and strategies for the control of transboundary and other economically important animal diseases in India: a Review. Indian J Anim Sci 75(4):456-464 Singh RK, Balamurugan V, Bhanuprakash V, Sen A, Saravanan P, Yadav MP (2009) Control and Eradication of peste des petits ruminants in sheep and goats in India: possibility. Vet Ital 45:449-462. Singh RP, De UK, Pandey KD (2010) Virological and antigenic characterization of two peste des petits ruminants (PPR) vacci

Shinto in Modern Japan

Shinto in Modern Japan Religion is a constant variable in today’s world as well as the past. In order to understand Shinto in modern Japan first Shinto must be looked at from the past. Native Japanese religion states Shinto is the way of the gods. Going into depth of Shinto history and the modern view of Shinto now will bring up where Shinto originated from, it’s comparison to other religions, and Shinto’s role in modern Japan. Like many main religions, Shinto originated from prehistoric times but is not truly known because it goes much too far back in time showing as far back as 720 A. D. Its name comes from Chinese words â€Å"shin-tao†. Native Japanese religion, Shinto, plays a very significant part in Japan’s society (Shinto2). Shinto is not only a religion but a way of living for the Japanese. This religion has made a permanent place in history around the world. Writer Chikao Fjisawa pronounced, â€Å"State Shinto – a system embodying nationalism loyalty and Emperor Veneration the Shinto was a â€Å"perversion of Shinto theory and beliefs into militaristic and ultra-nationalistic propaganda† (Boyd). State Shinto is known as the old Shinto. Just as any other religion, Shinto has been compared to other religions show close resemblance. Shinto has no defined dogma, scared scriptures, or ethical precepts. Japanese tend to combine Shinto with other religious beliefs like Buddhism and Christianity. They also tend not to attach just one of the religious beliefs giving each equal attention. Shrines called jinja is used to practice Shinto and has very distinct gates which make it easy to tell from Buddhist temples. The only reason Shinto was named and systemized in the 16th century was to mark the difference from Buddhism and Confucianism (Shinto1). After World War II a separation between government and Shinto took place. This separation was noted in the constitution and history. As history shows, the emperor issued a statement forbidding use of Shinto symbols as nationalistic reasons and renouncing all rights to divinity. Even in modern day, extremists still favor protests against these and other changes involving the Shinto. Today Shinto is still a strong practice. Many Japanese still use the Shinto shrines for marriage, or to bless a new child, car, and etc. Building, homes and other architectural plots are also known to be blessed for safety and protection. Hundreds of Shinto ceremonies are still carried out daily in today’s modern life such as festivals just naming one. In modern life the old Shinto is the main practice still being used. Main religions around the world have an unknown exact birth dates as well as playing an influence role in history. Shinto has not only made its mark in Japanese history, but still withstands in present day society. Religion tends to be a subject who holds controversy and mystery intriguing the minds of almost everyone at some point. Shinto’s origin may not be able to be definitely pinpointed but it’s very apparent that Shinto religion still stands very prominent in Japan’s modern life. As stated earlier, Shinto ceremonies take place daily in Japan and the religion is still practiced by a very large population of Japanese still today. Religion is truly a everlasting time capsule of knowledge telling bits and pieces of the world and relationships within the world. Works Cited Boyd, W. James and Williams, G. Ron â€Å"Reconsidering Shinto after World War II: Perspectives from the Life and Thought of a Shinto Priest. † . Motonisa, Yamakage. â€Å"The Essence of Shinto: Japan’s Spiritual Heart. † Kodansha America, Inc. , 2006. â€Å"Shinto. † . â€Å"Shinto. † .

Extinction of Certain Species

In the late century, extinction becomes common topic in our daily discussion and debates. An average of 27,000 species is currently extinct each year and there is a possibility of 22% of extinction in the overall species if action is not taken. Animal extinction is due to human immorality and irresponsible behavior just for their own benefits. Since the year 1600, a total of 83 mammals species are known to have become extinct. Wildlife population depleted the trade in live animals, damaged habitats and the countless animals of suffering. Wildlife International, 2008) Apart from that, animal is a good source to make traditional medicine. For example, Chinese believes that snake galls are good to cure diseases and strengthen immune system of the body. Sometimes, human thoughts are powerful which may kill many innocent animals. According to Jeanette McDermott, the book of Bear Muze stated that every year, approximately 10,000 Asiatic black bears are locked in cages the size of their own bodies just to have their bile drained and sold the use in traditional medicine.Besides, many ocean mammals are suffering due to the business benefits. For example, Chinese culture like to consume shark fin soup as part of the cuisine. Businessman will take the advantage to hunt shark fin for money because of its high demand. An organization devoted to preserve marine life, Ocean Environment, stated in Asian Geographic(Nov-Dec 2008) that shark fin soup prized as a delicacy in Chinese cuisine. It is becoming un-cool to consume because 90% of the sharks are un-finned while alive thrown into the sea.

U.s. Foreign Policy Of Containment - 1074 Words

-George Kennan George Kennan was a major factor in the U.S. foreign policy of containment, which was created during the Cold War in order to prevent the spread of Communist ideology of the Soviet Union in other countries. After World War II, George Kennan, â€Å"...a career diplomat and expert on Russia†¦Ã¢â‚¬ (Roark, Pg.867), had anticipated that the Soviet Union wanted to gain power and expand Communism throughout other countries, so in order to provide a counterforce and protect American capitalism, Kennan developed the idea of containment that defended threaten countries from Communist power with American atomic weapons, economic aid, espionage, propaganda, and army alliances. Eventually, the containment policy was significant in America and around the world because it was â€Å"...a critical turning point in the development of the Cold War, providing a compelling rationale for wielding U.S. power throughout the world.†(Roark, Pg.870) (Roark, Pg.867) -Keynesian Economics Keynesian economics, derived from the ideology of John Maynard Keynes’, was a strategy used during post World War II that would prevent economic decline in the United States by incorporating government spending. Keynesian economics would work by using â€Å"...deficit spending to stimulate the economy when in the down cycle and increased taxes to retire the debt during the upswing.†(Lecture A, Week 5). Some government spending programs that reflected the idea of Keynesian economics in America included The EmploymentShow MoreRelatedFrom The Dropping Of The Atomic Bombs On Japan In 19451498 Words   |  6 PagesStates of America has pursued a foreign policy of containment to curb the spread of Soviet communism throughout the world. The U.S. spent billions of dollars during the Cold War period on foreign policies of containment, but is criticized that these strategies were ineffective in containing the spread of communism. In 1946, The Long Telegram, put forth by the fat her of the containment theory, George F. 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